For High School Teachers
 
 
  From DNA to Organism: A Study in DNA Function for the High School Biology Classroom
 
 

Module 3: Manipulating genes

Explanation

          The students will be introduced to the idea that genes, like the one that we amplified, can be cut out and inserted into other DNA. The students will be introduced to the idea of gene splicing and gene cloning using bacterial hosts.  

Lectures: Manipulaton of genes: How does a scientist make a transgenic organism? Here we use a PowerPoint presentation about generating a transgenic organism; this presentation will be finished at a later date. We also use a commericially oint presentation about generating a transgenic organism; this presentation will be finished at a later date. We also use a commercially available kit to transform bacteria. The gene that is inserted into the target bacterial genome encodes a protein that causes the bacteria to glow. Hence, sucessfuly  transformation of the target organism can be easily observed.
Labs: We use the Bio-Rad pGlo transformation lab